Physical Mapping
Cytogenetic mapping (FISH, fiber FISH)
- Identification of low-copy BAC clones that can be used as molecular cytogenetic landmarks (IEB).
- Characterization of translocations by FISH of BAC clones anchored to a genetic map (CIRAD)
Gene discovery
- Screening of BAC libraries with markers for somaclonal variation to develop more extensive genomic context of these regions (CWRU)
- Screening of BAC libraries for disease-resistance related genes (CWRU)
- Screening of genomic BAC library of M. acuminata 'Calcutta4' for DNA clones free of repeated sequences. Selected BAC clones were localized to mitotic chromosomes by fluorescence in situ hybridization (FISH) (IEB)
- Analysis and characterization of M. balbisiana BAC clones containing BSV "integrants" (CIRAD)
- Development of gene primers for conserved genes related to anonymous genes, biotic and abiotic stress genes (UL)
- Selection of BAC clones of M. acuminata 'Calcutta 4' for comparative analysis of Musa and rice genome structure and organization (UCB, CIRAD, EMBRAPA, TIGR)
- Hybridization-based anchoring of more than 1200 gene probes designed for other monocots, to the M. acuminata BAC library, yielding about 13000 anchors (UGA)
- Identification of BACs carrying abiotic stress-related genes (UL)
- Identification of BACs carrying biotic stress-related genes (CIRAD, EMBRAPA, UCB)
- Comparative analysis of M. acuminata Calcutta 4, Cavendish, and M. balbisiana PKW BAC clones selected with NBS-LRR RGA probes (UCB)